@article{036c90ce13ed434187a76a138788f497,
title = "Photo-acceleration of protein release from endosome in the protein transduction system",
abstract = "The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system.",
keywords = "Endosomal release, FITC, Photo, Protein transduction, Protein transduction domain",
author = "Masayuki Matsushita and Hirofumi Noguchi and Lu, {Yun Fei} and Kazuhito Tomizawa and Hiroyuki Michiue and Li, {Sheng Tian} and Kenzo Hirose and Susan Bonner-Weir and Hideki Matsui",
note = "Funding Information: We thank Dr. T. Jacks (MIT) and Dr. H. Matsushita (Okayama U) for providing the glioma cell line. This research was supported by a Grant-in-Aid for Scientific Research on Priority Areas (C) “medical genome science” from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by NIH grants DK 61251, Research Foundation, the Diabetes Research and Wellness Foundation and an important group of private donors.",
year = "2004",
month = aug,
day = "13",
doi = "10.1016/j.febslet.2004.07.033",
language = "English",
volume = "572",
pages = "221--226",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",
}