TY - JOUR
T1 - Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan
AU - Takaishi, Asuka
AU - Kozhevnikov, Andrey E.
AU - Kozhevnikova, Zoya V.
AU - Ikeda, Hajime
AU - Fujii, Noriyuki
AU - Soejima, Akiko
N1 - Funding Information:
Sample collection would not have been possible without the help of many people, to whom we express our special thanks (alphabeti‐ cal order, title omitted): K. Hashiba, M. Igari, M. Inoue, K. Kato, T. Kawahara, S. Kinoshita, Y. Kokami, J. Nishihiro, J.‐H. Pak, T. Sato, K. Sawa, S. Sei, and K. Tanaka. We are grateful for the public institu‐ tions that granted us permission to collect samples. We also extend our appreciation for the members of our laboratory in supporting our research. This study was supported by a grant from the Institute of Plant Science and Resources, Okayama University for Soejima (2754 & 2835). We would like to thank Editage (www.editage.jp) for English language editing. Our cordial thanks are going to the referee who kindly gave us advices to revise our manuscript.
Publisher Copyright:
© 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
PY - 2019/6
Y1 - 2019/6
N2 - The genetic diversity and structure of Pulsatilla cernua, a continental-grassland relict, were investigated using variations in chloroplast DNA (cpDNA) and microsatellites of nuclear DNA. In the analyses of three cpDNA regions, 17 haplotypes were found in 24 populations of P. cernua from Japan, Korea, and Russia. Although the route and time of migration between the continent of Asia and Japan could not be well resolved, the cpDNA haplotype network suggests the existence of several ancient lineages in Japan and a recent secondary migration from Japan to the continent. Microsatellite analyses did not indicate genetic structure among the Japanese populations, indicating the existence of gene flow across the distribution area until recently. These results indicate that the present fragmentation of P. cernua in Japan may reflect a rapid, recent reduction from a previously large, continuous distribution.
AB - The genetic diversity and structure of Pulsatilla cernua, a continental-grassland relict, were investigated using variations in chloroplast DNA (cpDNA) and microsatellites of nuclear DNA. In the analyses of three cpDNA regions, 17 haplotypes were found in 24 populations of P. cernua from Japan, Korea, and Russia. Although the route and time of migration between the continent of Asia and Japan could not be well resolved, the cpDNA haplotype network suggests the existence of several ancient lineages in Japan and a recent secondary migration from Japan to the continent. Microsatellite analyses did not indicate genetic structure among the Japanese populations, indicating the existence of gene flow across the distribution area until recently. These results indicate that the present fragmentation of P. cernua in Japan may reflect a rapid, recent reduction from a previously large, continuous distribution.
KW - East Asia
KW - Japan
KW - Mansen plant
KW - continental-grassland relict
KW - phylogeography
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U2 - 10.1002/ece3.5298
DO - 10.1002/ece3.5298
M3 - Article
AN - SCOPUS:85069975133
SN - 2045-7758
VL - 9
SP - 7262
EP - 7272
JO - Ecology and Evolution
JF - Ecology and Evolution
IS - 12
ER -