Promotion of attachment of human bone marrow stromal cells by CCN2

Mitsuaki Ono; Satoshi Kubota; Takuo Fujisawa; Wataru Sonoyama; Harumi Kawaki; Kentaro Akiyama; Masamitsu Oshima; Takashi Nishida; Yasuhiro Yoshida; Kazuomi Suzuki; Masaharu Takigawa; Takuo Kuboki

doi:10.1016/j.bbrc.2007.03.052

Promotion of attachment of human bone marrow stromal cells by CCN2

Mitsuaki Ono, Satoshi Kubota, Takuo Fujisawa, Wataru Sonoyama, Harumi Kawaki, Kentaro Akiyama, Masamitsu Oshima, Takashi Nishida, Yasuhiro Yoshida, Kazuomi Suzuki, Masaharu Takigawa, Takuo Kuboki

研究成果 › 査読

18 被引用数 (Scopus)

抄録

Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin α_vβ₃ antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration.

本文言語	English
ページ（範囲）	20-25
ページ数	6
ジャーナル	Biochemical and Biophysical Research Communications
巻	357
号	1
DOI	https://doi.org/10.1016/j.bbrc.2007.03.052
出版ステータス	Published - 5月 25 2007

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

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10.1016/j.bbrc.2007.03.052

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title = "Promotion of attachment of human bone marrow stromal cells by CCN2",

abstract = "Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin αvβ3 antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration.",

keywords = "Bone marrow stromal cell, CCN2/connective tissue growth factor, Cell attachment, Hydroxyapatite, p38 MAPK",

author = "Mitsuaki Ono and Satoshi Kubota and Takuo Fujisawa and Wataru Sonoyama and Harumi Kawaki and Kentaro Akiyama and Masamitsu Oshima and Takashi Nishida and Yasuhiro Yoshida and Kazuomi Suzuki and Masaharu Takigawa and Takuo Kuboki",

note = "Funding Information: This study was supported by Grants-in-Aid for scientific research (A) (to T.K.), (S) (to M.T.), and C (to S.K. and T.F.) from Japan Society for the Promotion of Science. We thank Drs. Takako Hattori and Seiji Kondo for helpful suggestions. ",

year = "2007",

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doi = "10.1016/j.bbrc.2007.03.052",

language = "English",

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T1 - Promotion of attachment of human bone marrow stromal cells by CCN2

AU - Ono, Mitsuaki

AU - Kubota, Satoshi

AU - Fujisawa, Takuo

AU - Sonoyama, Wataru

AU - Kawaki, Harumi

AU - Akiyama, Kentaro

AU - Oshima, Masamitsu

AU - Nishida, Takashi

AU - Yoshida, Yasuhiro

AU - Suzuki, Kazuomi

AU - Takigawa, Masaharu

AU - Kuboki, Takuo

N1 - Funding Information: This study was supported by Grants-in-Aid for scientific research (A) (to T.K.), (S) (to M.T.), and C (to S.K. and T.F.) from Japan Society for the Promotion of Science. We thank Drs. Takako Hattori and Seiji Kondo for helpful suggestions.

PY - 2007/5/25

Y1 - 2007/5/25

N2 - Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin αvβ3 antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration.

AB - Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin αvβ3 antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration.

KW - Bone marrow stromal cell

KW - CCN2/connective tissue growth factor

KW - Cell attachment

KW - Hydroxyapatite

KW - p38 MAPK

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Promotion of attachment of human bone marrow stromal cells by CCN2

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