Quantitative nature of overexpression experiments

研究成果査読

86 被引用数 (Scopus)

抄録

Overexpression experiments are sometimes considered as qualitative experiments designed to identify novel proteins and study their function. However, in order to draw conclusions regarding protein overexpression through association analyses using large-scale biological data sets, we need to recognize the quantitative nature of overexpression experiments. Here I discuss the quantitative features of two different types of overexpression experiment: absolute and relative. I also introduce the four primary mechanisms involved in growth defects caused by protein overexpression: resource overload, stoichiometric imbalance, promiscuous interactions, and pathway modulation associated with the degree of overexpression.

本文言語English
ページ(範囲)3932-3939
ページ数8
ジャーナルMolecular Biology of the Cell
26
22
DOI
出版ステータスPublished - 11月 5 2015

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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