TY - JOUR
T1 - Red blood cell coagulation induced by low-temperature plasma treatment
AU - Miyamoto, Kenji
AU - Ikehara, Sanae
AU - Takei, Hikaru
AU - Akimoto, Yoshihiro
AU - Sakakita, Hajime
AU - Ishikawa, Kenji
AU - Ueda, Masashi
AU - Ikeda, Jun-Ichiro
AU - Yamagishi, Masahiro
AU - Kim, Jaeho
AU - Yamaguchi, Takashi
AU - Nakanishi, Hayao
AU - Shimizu, Tetsuji
AU - Shimizu, Nobuyuki
AU - Hori, Masaru
AU - Ikehara, Yuzuru
N1 - Copyright © 2016 Elsevier Inc. All rights reserved.
PY - 2016/3/28
Y1 - 2016/3/28
N2 - Low-temperature plasma (LTP) treatment promotes blood clot formation by stimulation of the both platelet aggregation and coagulation factors. However, the appearance of a membrane-like structure in clots after the treatment is controversial. Based on our previous report that demonstrated characteristics of the form of coagulation of serum proteins induced by LTP treatment, we sought to determine whether treatment with two plasma instruments, namely BPC-HP1 and PN-110/120TPG, formed clots only from red blood cells (RBCs). LTP treatment with each device formed clots from whole blood, whereas LTP treatment with BPC-HP1 formed clots in phosphate-buffered saline (PBS) containing 2 × 10(9)/mL RBCs. Light microscopic analysis results showed that hemolysis formed clots consisting of materials with membrane-like structures from both whole blood and PBS-suspended RBCs. Moreover, electron microscopic analysis results showed a monotonous material with high electron density in the formed clots, presenting a membrane-like structure. Hemolysis disappeared with the decrease in the current through the targets contacting with the plasma flare and clot formation ceased. Taken together, our results and those of earlier studies present two types of blood clot formation, namely presence or absence of hemolysis capability depending on the current through the targets.
AB - Low-temperature plasma (LTP) treatment promotes blood clot formation by stimulation of the both platelet aggregation and coagulation factors. However, the appearance of a membrane-like structure in clots after the treatment is controversial. Based on our previous report that demonstrated characteristics of the form of coagulation of serum proteins induced by LTP treatment, we sought to determine whether treatment with two plasma instruments, namely BPC-HP1 and PN-110/120TPG, formed clots only from red blood cells (RBCs). LTP treatment with each device formed clots from whole blood, whereas LTP treatment with BPC-HP1 formed clots in phosphate-buffered saline (PBS) containing 2 × 10(9)/mL RBCs. Light microscopic analysis results showed that hemolysis formed clots consisting of materials with membrane-like structures from both whole blood and PBS-suspended RBCs. Moreover, electron microscopic analysis results showed a monotonous material with high electron density in the formed clots, presenting a membrane-like structure. Hemolysis disappeared with the decrease in the current through the targets contacting with the plasma flare and clot formation ceased. Taken together, our results and those of earlier studies present two types of blood clot formation, namely presence or absence of hemolysis capability depending on the current through the targets.
U2 - 10.1016/j.abb.2016.03.023
DO - 10.1016/j.abb.2016.03.023
M3 - Article
C2 - 27033148
SN - 0003-9861
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
ER -