Silica gel macrospheres were prepared from water glass as the silica source. The water glass solution was mixed with sodium alginate solution and added dropwisely to 1M CaCl2 solution to form macrospheres with 34 and 2.6mm in diameter. Those SiO2 macrospheres were soaked in 1:1 (volume) mixture of ethanol and 0.1M Na2HPO4 to deposit hydroxyapatite layer (HAp-SiO2 macrospheres). Bovine serum albumin (BSA) and egg lysozyme (LYZ) were adsorbed those SiO2 and HAp-SiO2 macrospheres. The amount of proteins adsorbed was well correlated to the Langmuir-type adsorption equation. The electrostatic interaction was predominant between the macrosphere surface and the protein molecules. Protein molecular entrapment in the mesopores (5-25 nm) was also probable for the SiO2 macrospheres but not applicable to the HAp-SiO2 ones without the mesopores. From consideration that protein molecule has a few groups with negative or positive partial charges, a multi-layer adsorption model was proposed.
|ジャーナル||Journal of the Ceramic Society of Japan|
|出版ステータス||Published - 9月 2012|
ASJC Scopus subject areas
- 化学 (全般)