TY - JOUR
T1 - Spontaneous Structural Changes in Actin Regulate G-F Transformation
AU - Morimatsu, Masatoshi
AU - Togashi, Yuichi
AU - Nishikawa, So
AU - Sugawa, Mitsuhiro
AU - Iwane, Atsuko H.
AU - Yanagida, Toshio
PY - 2012/11/5
Y1 - 2012/11/5
N2 - Transformations between G- (monomeric) and F-actin (polymeric) are important in cellular behaviors such as migration, cytokinesis, and morphing. In order to understand these transitions, we combined single-molecule Förster resonance energy transfer with total internal reflection fluorescence microscopy to examine conformational changes of individual actin protomers. We found that the protomers can take different conformational states and that the transition interval is in the range of hundreds of seconds. The distribution of these states was dependent on the environment, suggesting that actin undergoes spontaneous structural changes that accommodate itself to polymerization.
AB - Transformations between G- (monomeric) and F-actin (polymeric) are important in cellular behaviors such as migration, cytokinesis, and morphing. In order to understand these transitions, we combined single-molecule Förster resonance energy transfer with total internal reflection fluorescence microscopy to examine conformational changes of individual actin protomers. We found that the protomers can take different conformational states and that the transition interval is in the range of hundreds of seconds. The distribution of these states was dependent on the environment, suggesting that actin undergoes spontaneous structural changes that accommodate itself to polymerization.
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U2 - 10.1371/journal.pone.0045864
DO - 10.1371/journal.pone.0045864
M3 - Article
C2 - 23144776
AN - SCOPUS:84868374651
SN - 1932-6203
VL - 7
JO - PLoS One
JF - PLoS One
IS - 11
M1 - e45864
ER -