TY - CHAP
T1 - State transition mechanism in arabidopsis thaliana
T2 - Biophysical and proteomic studies
AU - Nellaepalli, Sreedhar
AU - Zsiros, Ottó
AU - Kovács, László
AU - Venkateswarlu, Yadavalli
AU - Rao, Mekala Nageswara
AU - Mohanty, Prasanna
AU - Subramanyam, Rajagopal
N1 - Publisher Copyright:
© Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2013.
PY - 2013
Y1 - 2013
N2 - The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state II and vice versa. The redox state of the QA, the primary acceptor of photosystem II (PSII) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chl afluorescence transient studies revealed that in state II, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSII. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSI under state II condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSII. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state II this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state II. The additional spot is yet to be investigated.
AB - The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state II and vice versa. The redox state of the QA, the primary acceptor of photosystem II (PSII) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chl afluorescence transient studies revealed that in state II, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSII. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSI under state II condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSII. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state II this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state II. The additional spot is yet to be investigated.
KW - 2D electrophoresis
KW - Arabidopsis thaliana
KW - Chl a fluorescence
KW - Photosystems
KW - State transition
UR - https://www.scopus.com/pages/publications/85060718500
UR - https://www.scopus.com/pages/publications/85060718500#tab=citedBy
U2 - 10.1007/978-3-642-32034-7_83
DO - 10.1007/978-3-642-32034-7_83
M3 - Chapter
AN - SCOPUS:85060718500
T3 - Advanced Topics in Science and Technology in China
SP - 398
EP - 401
BT - Advanced Topics in Science and Technology in China
PB - Springer Science and Business Media Deutschland GmbH
ER -