@article{8c2fef8393024111ba4240074112e751,
title = "Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity",
abstract = "Vesicle-inducing protein in plastids 1 (VIPP1) is essential for the biogenesis and maintenance of thylakoid membranes, which transform light into life. However, it is unknown how VIPP1 performs its vital membrane-remodeling functions. Here, we use cryo-electron microscopy to determine structures of cyanobacterial VIPP1 rings, revealing how VIPP1 monomers flex and interweave to form basket-like assemblies of different symmetries. Three VIPP1 monomers together coordinate a non-canonical nucleotide binding pocket on one end of the ring. Inside the ring's lumen, amphipathic helices from each monomer align to form large hydrophobic columns, enabling VIPP1 to bind and curve membranes. In vivo mutations in these hydrophobic surfaces cause extreme thylakoid swelling under high light, indicating an essential role of VIPP1 lipid binding in resisting stress-induced damage. Using cryo-correlative light and electron microscopy (cryo-CLEM), we observe oligomeric VIPP1 coats encapsulating membrane tubules within the Chlamydomonas chloroplast. Our work provides a structural foundation for understanding how VIPP1 directs thylakoid biogenesis and maintenance.",
keywords = "CLEM, Chlamydomonas, ESCRT-III, Synechocystis, cryo-EM, cryo-electron tomography, membrane remodeling, nucleotide hydrolysis, photosynthesis, stress response, thylakoid biogenesis",
author = "Gupta, {Tilak Kumar} and Sven Klumpe and Karin Gries and Steffen Heinz and Wojciech Wietrzynski and Norikazu Ohnishi and Justus Niemeyer and Benjamin Spaniol and Miroslava Schaffer and Anna Rast and Matthias Ostermeier and Mike Strauss and Plitzko, {J{\"u}rgen M.} and Wolfgang Baumeister and Till Rudack and Wataru Sakamoto and J{\"o}rg Nickelsen and Schuller, {Jan M.} and Michael Schroda and Engel, {Benjamin D.}",
note = "Funding Information: We thank Conrad Mullineaux for generously providing the Synechocystis vipp1-GFP strain; Elisabeth Weyher-Stingl and Victoria Sanchez Caballero at the MPI Biochemistry core facility for assistance with mass spectrometry; Ricardo Righetto for performing data deposition; G{\"u}nter Pfeifer and Florian Beck for help with microscopy and image analysis; Alexander Hertle, Ralph Bock, Christin Radon, and Petra Wendler for assistance with CLEM experiments; Gert Bange for helpful discussions; and Karin Engel for critically reading the manuscript. J.M.P. thanks Leica for a collaboration to develop the prototype cryo-confocal microscope. This work was supported by grants from the Deutsche Forschungsgemeinschaft to J.N. ( NI 390/9-2 as part of FOR2092 ), J.M.S. (Emmy Noether grant SCHU 3364/1-1 ), M.S. ( Schr 617/8-2 as part of FOR2092 and TRR 175 project C02 ), and B.D.E. ( EN 1194/1-1 as part of FOR2092 ), as well as KAKENHI grants to W.S. ( 16H06554 from the Ministry of Education, Culture, Sports, Science and Technology and 21H02508 from the Japanese Society for the Promotion of Science ). Additional funding was provided by the Max Planck Society , the Helmholtz Zentrum M{\"u}nchen , and LMU Munich {\textquoteright}s Institutional Strategy “LMU Excellent” within the framework of the German Excellence Initiative . Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jul,
day = "8",
doi = "10.1016/j.cell.2021.05.011",
language = "English",
volume = "184",
pages = "3643--3659.e23",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "14",
}