TY - JOUR
T1 - Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits
AU - Qin, Xiaochun
AU - Pi, Xiong
AU - Wang, Wenda
AU - Han, Guangye
AU - Zhu, Lixia
AU - Liu, Mingmei
AU - Cheng, Linpeng
AU - Shen, Jian Ren
AU - Kuang, Tingyun
AU - Sui, Sen Fang
N1 - Funding Information:
We thank J. Lei and the staff at the Tsinghua University Branch of the National Center for Protein Sciences Beijing for providing facility support, and the Explorer 100 cluster system of the Tsinghua National Laboratory for Information Science and Technology for providing computation resources. We thank S. Qin and Z. Liu from Yantai Institute of Coastal Zone Research, CAS for assistance in collecting B. corticulans, L. Shu from Shanghai Luming Biotechnology for mass spectrometry analysis. The project was funded by the National Key R&D Program of China (2017YFA0503700, 2016YFA0501101, 2015CB150101); the National Natural Science Foundation of China (31622007, 31670237, 31600191); a Strategic Priority Research Program of CAS (XDB17000000); State Key Laboratory of Membrane Biology; and Taishan Scholars Project.
Publisher Copyright:
© 2019, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Photosystem I (PSI) is a highly efficient natural light-energy converter, and has diverse light-harvesting antennas associated with its core in different photosynthetic organisms. In green algae, an extremely large light-harvesting complex I (LHCI) captures and transfers energy to the PSI core. Here, we report the structure of PSI–LHCI from a green alga Bryopsis corticulans at 3.49 Å resolution, obtained by single-particle cryo-electron microscopy, which revealed 13 core subunits including subunits characteristic of both prokaryotes and eukaryotes, and 10 light-harvesting complex a (Lhca) antennas that form a double semi-ring and an additional Lhca dimer, including a novel four-transmembrane-helix Lhca. In total, 244 chlorophylls were identified, some of which were located at key positions for the fast energy transfer. These results provide a firm structural basis for unravelling the mechanisms of light-energy harvesting, transfer and quenching in the green algal PSI–LHCI, and important clues as to how PSI–LHCI has changed during evolution.
AB - Photosystem I (PSI) is a highly efficient natural light-energy converter, and has diverse light-harvesting antennas associated with its core in different photosynthetic organisms. In green algae, an extremely large light-harvesting complex I (LHCI) captures and transfers energy to the PSI core. Here, we report the structure of PSI–LHCI from a green alga Bryopsis corticulans at 3.49 Å resolution, obtained by single-particle cryo-electron microscopy, which revealed 13 core subunits including subunits characteristic of both prokaryotes and eukaryotes, and 10 light-harvesting complex a (Lhca) antennas that form a double semi-ring and an additional Lhca dimer, including a novel four-transmembrane-helix Lhca. In total, 244 chlorophylls were identified, some of which were located at key positions for the fast energy transfer. These results provide a firm structural basis for unravelling the mechanisms of light-energy harvesting, transfer and quenching in the green algal PSI–LHCI, and important clues as to how PSI–LHCI has changed during evolution.
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U2 - 10.1038/s41477-019-0379-y
DO - 10.1038/s41477-019-0379-y
M3 - Article
C2 - 30850820
AN - SCOPUS:85062611408
SN - 2055-026X
VL - 5
SP - 263
EP - 272
JO - Nature Plants
JF - Nature Plants
IS - 3
ER -