TY - JOUR
T1 - Structures of N-Linked Oligosaccharides of Microsomal Glycoproteins from Developing Castor Bean Endosperms
AU - Kimura, Yoshinobu
AU - Tokuda, Toshihiko
AU - Yamai, Masafumi
AU - Nakajima, Shuhei
AU - Higashide, Eiji
AU - Takagi, Shigeaki
N1 - Funding Information:
t This work was supported in part by a Grant-in-Aid for Encouragement of Young Scientists (No. 03760055) from the Ministry of Education, Science, and Culture of Japan. tt To whom correspondence should be addressed. Abbreviations: PA-, pyridylamino; RCA, Ricinus communis agglutinin; RER, rough endoplasmic reticulum; Man, D-mannose; Xyl, D-xyiose;
PY - 1992
Y1 - 1992
N2 - The structures of sugar chains of the glycoproteins from the microsomal fraction of developing castor bean endosperms have been analyzed. The structural analyses were done by a fluorescence method combined with component analysis, exoglycosidase digestions, partial acetolysis, Smith degradation, and 1H-NMR spectroscopy. The estimated structures fell into three categories; the first was oligomannose-type, the second xylomannose-type, the third complex-type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2 (M3FX) and Man6GlcNAc2 (M6B) were the major structures. The structures of Man4GlcNAc2 (M4C) and Man4Xyl1GlcNAc2 (M4X) have also been found in the microsomal glycoproteins of the developing bean endosperms. These results could indicate that the structures of M4C, M4X, and M3FX are formed in the stage of sugar chain processing in the microsomal fraction, in which oligomannose-type sugar chains are modified into complex-type ones by several kinds of processing enzymes.
AB - The structures of sugar chains of the glycoproteins from the microsomal fraction of developing castor bean endosperms have been analyzed. The structural analyses were done by a fluorescence method combined with component analysis, exoglycosidase digestions, partial acetolysis, Smith degradation, and 1H-NMR spectroscopy. The estimated structures fell into three categories; the first was oligomannose-type, the second xylomannose-type, the third complex-type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2 (M3FX) and Man6GlcNAc2 (M6B) were the major structures. The structures of Man4GlcNAc2 (M4C) and Man4Xyl1GlcNAc2 (M4X) have also been found in the microsomal glycoproteins of the developing bean endosperms. These results could indicate that the structures of M4C, M4X, and M3FX are formed in the stage of sugar chain processing in the microsomal fraction, in which oligomannose-type sugar chains are modified into complex-type ones by several kinds of processing enzymes.
UR - http://www.scopus.com/inward/record.url?scp=0026810855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026810855&partnerID=8YFLogxK
U2 - 10.1271/bbb.56.215
DO - 10.1271/bbb.56.215
M3 - Article
C2 - 1368297
AN - SCOPUS:0026810855
SN - 0916-8451
VL - 56
SP - 215
EP - 222
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 2
ER -