Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)

Shoji Sonoda; Hisaaki Tsumuki

doi:10.1016/j.pestbp.2005.01.003

Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)

Shoji Sonoda
, Hisaaki Tsumuki

研究成果 › 査読

51 被引用数 (Scopus)

抄録

Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.

本文言語	English
ページ（範囲）	94-101
ページ数	8
ジャーナル	Pesticide Biochemistry and Physiology
巻	82
号	1
DOI	https://doi.org/10.1016/j.pestbp.2005.01.003
出版ステータス	Published - 5月 2005
外部発表	はい

ASJC Scopus subject areas

農業および作物学
健康、毒物学および変異誘発

文献へのアクセス

10.1016/j.pestbp.2005.01.003

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引用スタイル

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title = "Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)",

abstract = "Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.",

keywords = "Chlorfluazuron, Diamondback moth, Glutathione S-transferase, Insecticide resistance, Plutella xylostella L.",

author = "Shoji Sonoda and Hisaaki Tsumuki",

note = "Funding Information: This study was supported in part by the Wesco Scientific Promotion Foundation, the Ohara Foundation, and a Grant-in-Aid for Encouragement of Young Scientists from the Japan Society for the Promotion of Science and Technology (JSPS) (No. 16780036). The authors thank Dr. T. Miyata (Nagoya University, Japan) and Ms. T. Takeda (Nagoya University) for advice regarding GST assays; Dr. M. Ashfaq (Okayama University, Japan) for reading the manuscript. ",

year = "2005",

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AU - Sonoda, Shoji

AU - Tsumuki, Hisaaki

N1 - Funding Information: This study was supported in part by the Wesco Scientific Promotion Foundation, the Ohara Foundation, and a Grant-in-Aid for Encouragement of Young Scientists from the Japan Society for the Promotion of Science and Technology (JSPS) (No. 16780036). The authors thank Dr. T. Miyata (Nagoya University, Japan) and Ms. T. Takeda (Nagoya University) for advice regarding GST assays; Dr. M. Ashfaq (Okayama University, Japan) for reading the manuscript.

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N2 - Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.

AB - Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.

KW - Chlorfluazuron

KW - Diamondback moth

KW - Glutathione S-transferase

KW - Insecticide resistance

KW - Plutella xylostella L.

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