TY - JOUR
T1 - Surface plasmon resonance biosensor with high anti-fouling ability for the detection of cardiac marker troponin T
AU - Liu, Jen Tsai
AU - Chen, Ching Jung
AU - Ikoma, Toshiyuki
AU - Yoshioka, Tomohiko
AU - Cross, Jeffrey S.
AU - Chang, Shwu Jen
AU - Tsai, Jang Zern
AU - Tanaka, Junzo
N1 - Funding Information:
This study was supported by G-COE short-term visit program on “Education and Research Center for Material Innovation”, at Tokyo Institute of Technology, Tokyo, Japan, and the National Science Council, Taiwan ( NSC 99-2627-E-214-001 ).
PY - 2011/10/3
Y1 - 2011/10/3
N2 - Designing a surface recognition layer with high anti-fouling ability, high affinity, and high specificity is an important issue to produce high sensitivity biosensing transducers. In this study, a self-assembled monolayer (SAM) consisting of a homogeneous mixture of oligo(ethylene glycol) (OEG)-terminated alkanethiolate and mercaptohexadecanoic acid (MHDA) on Au was employed for immobilizing troponin T antibody and applied in detecting cardiac troponin T by using surface plasmon resonance (SPR). The mixed SAM showed no phase segregation and exhibited human serum albumin resistance, particularly with an antibody-immobilized surface. X-ray photoemission spectra revealed that the chemical composition ratio of OEG to the mixed SAM was 69% and the OEG packing density was 82%. The specific binding of troponin T on the designed surface indicated a good linear correlation (R=0.991, P<0.0009) at concentrations lower than 50μgmL -1 with the limit of detection of 100ngmL -1 using a SPR measuring instrument. It is concluded that the mixed SAM functions as designed since it has high detection capability, high accuracy and reproducibility, as well as shows strong potential to be applied in rapid clinical diagnosis for label-free detection within 2min.
AB - Designing a surface recognition layer with high anti-fouling ability, high affinity, and high specificity is an important issue to produce high sensitivity biosensing transducers. In this study, a self-assembled monolayer (SAM) consisting of a homogeneous mixture of oligo(ethylene glycol) (OEG)-terminated alkanethiolate and mercaptohexadecanoic acid (MHDA) on Au was employed for immobilizing troponin T antibody and applied in detecting cardiac troponin T by using surface plasmon resonance (SPR). The mixed SAM showed no phase segregation and exhibited human serum albumin resistance, particularly with an antibody-immobilized surface. X-ray photoemission spectra revealed that the chemical composition ratio of OEG to the mixed SAM was 69% and the OEG packing density was 82%. The specific binding of troponin T on the designed surface indicated a good linear correlation (R=0.991, P<0.0009) at concentrations lower than 50μgmL -1 with the limit of detection of 100ngmL -1 using a SPR measuring instrument. It is concluded that the mixed SAM functions as designed since it has high detection capability, high accuracy and reproducibility, as well as shows strong potential to be applied in rapid clinical diagnosis for label-free detection within 2min.
KW - Anti-fouling ability
KW - Myocardial infarction
KW - Oligoethylene glycol
KW - Self-assembled monolayer
KW - Surface plasmon resonance
KW - Troponin T
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U2 - 10.1016/j.aca.2011.07.019
DO - 10.1016/j.aca.2011.07.019
M3 - Article
C2 - 21843678
AN - SCOPUS:80051668326
SN - 0003-2670
VL - 703
SP - 80
EP - 86
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -