@article{613b3f09f4374336ae2ed7fddc01959a,
title = "Targeted genome modifications in cereal crops",
abstract = "The recent advent of customizable endonucleases has led to remarkable advances in genetic engineering, as these molecular scissors allow for the targeted introduction of mutations or even precisely predefined genetic modifications into virtually any genomic target site of choice. Thanks to its unprecedented precision, effi-ciency, and functional versatility, this technology, commonly referred to as genome editing, has become an effective force not only in basic research devoted to the elucidation of gene function, but also for knowledge-based improvement of crop traits. Among the different platforms currently available for site-directed genome modifications, RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) endonucleases have proven to be the most powerful. This review provides an application-oriented overview of the development of customizable endonucleases, current approaches to cereal crop breeding, and future opportunities in this field.",
keywords = "barley, CRISPR, Maize, Rice, TALEN, Wheat, Zinc-finger nucleases",
author = "Hiroshi Hisano and Fumitaka Abe and Hoffie, {Robert E.} and Jochen Kumlehn",
note = "Funding Information: HH and JK were supported by the Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University (FY2017). HH and FA were supported by the Cabinet Office, Government of Japan, Cross-ministerial Strategic Innovation Promotion Program (SIP), ?Technolo-gies for Creating Next-generation Agriculture, Forestry and Fisheries? (funding agency: Bio-oriented Technology Research Advancement Institution, NARO). RH is grateful to the German Ministry of Research and Education (BMBF) for financial support in the context of the IDEMODERESBAR project (FKZ 031B0199C and 031B0887C). HH is supported by JSPS KAKENHI Grant-in-Aid for Scientific Research (B) (Grant Number JP19H02930). Publisher Copyright: {\textcopyright} 2021, Japanese Society of Breeding. All rights reserved.",
year = "2021",
month = oct,
doi = "10.1270/jsbbs.21019",
language = "English",
volume = "71",
pages = "405--416",
journal = "Breeding Science",
issn = "1344-7610",
publisher = "Japanese Society of Breeding",
number = "4",
}