The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process

Anna Ochi, Koki Makabe, Ryota Yamagami, Akira Hirata, Reiko Sakaguchi, Ya Ming Hou, Kazunori Watanabe, Osamu Nureki, Kunihiro Kuwajima, Hiroyuki Hori

研究成果査読

28 被引用数 (Scopus)

抄録

Background: Topologically knotted tRNA methyltransferases specifically recognize substrate tRNA. Results: Site-directed mutagenesis studies, chimeric protein analysis, and pre-steady state kinetics clarify the tRNA recognition sites of TrmH. Conclusion: The N- and C-terminal regions function in the initial binding process, and substrate tRNA is discriminated by the catalytic domain in an induced-fit process. Significance: Study of how proteins recognize RNA is crucial for understanding RNA maturation processes.

本文言語English
ページ(範囲)25562-25574
ページ数13
ジャーナルJournal of Biological Chemistry
288
35
DOI
出版ステータスPublished - 8月 30 2013

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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