The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs

A. Tezvergil-Mutluay, K. A. Agee, T. Uchiyama, S. Imazato, M. M. Mutluay, M. Cadenaro, L. Breschi, Y. Nishitani, F. R. Tay, D. H. Pashley

研究成果査読

144 被引用数 (Scopus)

抄録

Matrix metalloproteinases (MMPs) bound to dentin contribute to the progressive degradation of collagen fibrils in hybrid layers created by dentin adhesives. This study evaluated the MMP-inhibiting potential of quaternary ammonium methacrylates (QAMs), with soluble rhMMP-9 and a matrix-bound endogenous MMP model. Six different QAMs were initially screened by a rhMMP-9 colorimetric assay. For the matrix-bound endogenous MMPs, we aged demineralized dentin beams for 30 days in calcium- and zinc-containing media (CM; control), chlorhexidine, or QAMs in CM to determine the changes in dry mass loss and solubilization of collagen peptides against baseline levels. The inhibitory effects of QAMs on soluble rhMMP-9 varied between 34 and 100%. Beams incubated in CM showed a 29% decrease in dry mass (p < 0.05), whereas beams incubated with QAMs showed only 0.2%-6% loss of dry mass. Significantly more solubilized collagen was detected from beams incubated in CM (p < 0.05). It is concluded that QAMs exhibited dentin MMP inhibition comparable with that of chlorhexidine, but required higher concentrations.

本文言語English
ページ(範囲)535-540
ページ数6
ジャーナルJournal of dental research
90
4
DOI
出版ステータスPublished - 4月 2011
外部発表はい

ASJC Scopus subject areas

  • 歯学一般

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