The lattice-like structure observed by Vipp1-GFP in arabidopsis chloroplasts

Lingang Zhang, Yusuke Kato, Koji Saigo, Ute C. Vothknecht, Wataru Sakamoto

研究成果

抄録

Vipp1 (vesicle inducing protein in plastids 1) is proposed to play a role in thylakoid biogenesis. It is closely related to PspA (phage shock protein A), a bacterial protein that is induced under stress conditions. Despite its discovery a decade ago and extensive analysis in cyanobacteria, green algae and higher plants, the precise role of Vipp1 in the process of chloroplast development remains unclear. In this research, we expressed Vipp1 C-terminally fused to GFP (Vipp1-GFP) in Arabidopsis and found that Vipp1 is able to assemble into rod-shaped supercomplexes. Vipp1-GFP can rescue heterotrophic growth of a vipp1 knock-down mutant, suggesting that it complements Vipp1 function. Interestingly, Vipp1-GFP rods always appeared to cross with each other to form a lattice-like structure, which is similar to a scaffold structure formed by PspA in Escherichia coli. Based on these results, we infer that Vipp1 is involved in not only thylakoid biogenesis but chloroplast envelope integrity.

本文言語English
ホスト出版物のタイトルAdvanced Topics in Science and Technology in China
出版社Springer Science and Business Media Deutschland GmbH
ページ394-397
ページ数4
DOI
出版ステータスPublished - 2013

出版物シリーズ

名前Advanced Topics in Science and Technology in China
ISSN(印刷版)1995-6819
ISSN(電子版)1995-6827

ASJC Scopus subject areas

  • 化学工学(全般)
  • 工学(全般)
  • 一般

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