The Ras target AF-6 is a substrate of the fam deubiquitinating enzyme

Shinichiro Taya, Takaharu Yamamoto, Kyoko Kano, Yoji Kawano, Akihiro Iwamatsu, Tomoko Tsuchiya, Keiji Tanaka, Masami Kanai-Azuma, Stephen A. Wood, John S. Mattick, Kozo Kaibuchi


103 被引用数 (Scopus)


The Ras target AF-6 has been shown to serve as one of the peripheral components of cell-cell adhesions, and is thought to participate in cell- cell adhesion regulation downstream of Ras. We here purified an AF-6- interacting protein with a molecular mass of ~220 kD (p220) to investigate the function of AF-6 at cell-cell adhesions. The peptide sequences of p220 were identical to the amino acid sequences of mouse Fam. Fam is homologous to a deubiquitinating enzyme in Drosophila, the product of the fat facets gene. Recent genetic analyses indicate that the deubiquitinating activity of the fat facets product plays a critical role in controlling the cell fate. We found that Fam accumulated at the cell-cell contact sites of MDCKII cells, but not at free ends of plasma membranes. Fam was partially colocalized with AF-6 and interacted with AF-6 in vivo and in vitro. We also showed that AF-6 was ubiquitinated in intact cells, and that Fam prevented the ubiquitination of AF-6. These results indicate that AF-6 forms a complex with and serves as one of the substrates for Fam, and suggest that the degradation of peripheral components of cell-cell adhesions may be regulated by Fam.

ジャーナルJournal of Cell Biology
出版ステータスPublished - 8月 24 1998

ASJC Scopus subject areas

  • 細胞生物学


「The Ras target AF-6 is a substrate of the fam deubiquitinating enzyme」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。