TY - JOUR
T1 - The role of bone marrow-derived cells during the bone healing process in the GFP mouse bone marrow transplantation model
AU - Tsujigiwa, Hidetsugu
AU - Hirata, Yasuhisa
AU - Katase, Naoki
AU - Buery, Rosario Rivera
AU - Tamamura, Ryo
AU - Ito, Satoshi
AU - Takagi, Shin
AU - Iida, Seiji
AU - Nagatsuka, Hitoshi
N1 - Funding Information:
This work was partially supported by Grants-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, to H.T. (20791515), N.K. (22791766), R.T. (24592766), and H.N. (24659891).
PY - 2013/3
Y1 - 2013/3
N2 - Bone healing is a complex and multistep process in which the origin of the cells participating in bone repair is still unknown. The involvement of bone marrow-derived cells in tissue repair has been the subject of recent studies. In the present study, bone marrow-derived cells in bone healing were traced using the GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) were transplanted into C57BL/6 J wild mice. After transplantation, bone injury was created using a 1.0-mm drill. Bone healing was histologically assessed at 3, 7, 14, and 28 postoperative days. Immunohistochemistry for GFP; double-fluorescent immunohistochemistry for GFP-F4/80, GFP-CD34, and GFP-osteocalcin; and double-staining for GFP and tartrate-resistant acid phosphatase were performed. Bone marrow transplantation successfully replaced the hematopoietic cells into GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts or osteocytes in the repair stage were GFP-negative, whereas osteoclasts in the repair and remodeling stages and hematopoietic cells were GFP-positive. The results indicated that bone marrow-derived cells might not differentiate into osteoblasts. The role of bone marrow-derived cells might be limited to adjustment of the microenvironment by differentiating into inflammatory cells, osteoclasts, or endothelial cells in immature blood vessels.
AB - Bone healing is a complex and multistep process in which the origin of the cells participating in bone repair is still unknown. The involvement of bone marrow-derived cells in tissue repair has been the subject of recent studies. In the present study, bone marrow-derived cells in bone healing were traced using the GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) were transplanted into C57BL/6 J wild mice. After transplantation, bone injury was created using a 1.0-mm drill. Bone healing was histologically assessed at 3, 7, 14, and 28 postoperative days. Immunohistochemistry for GFP; double-fluorescent immunohistochemistry for GFP-F4/80, GFP-CD34, and GFP-osteocalcin; and double-staining for GFP and tartrate-resistant acid phosphatase were performed. Bone marrow transplantation successfully replaced the hematopoietic cells into GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts or osteocytes in the repair stage were GFP-negative, whereas osteoclasts in the repair and remodeling stages and hematopoietic cells were GFP-positive. The results indicated that bone marrow-derived cells might not differentiate into osteoblasts. The role of bone marrow-derived cells might be limited to adjustment of the microenvironment by differentiating into inflammatory cells, osteoclasts, or endothelial cells in immature blood vessels.
KW - Bone fracture
KW - Bone healing
KW - Bone marrow transplantation
KW - Bone marrow-derived cell
KW - GFP
UR - https://www.scopus.com/pages/publications/84873738285
UR - https://www.scopus.com/pages/publications/84873738285#tab=citedBy
U2 - 10.1007/s00223-012-9685-3
DO - 10.1007/s00223-012-9685-3
M3 - Article
C2 - 23263655
AN - SCOPUS:84873738285
SN - 0171-967X
VL - 92
SP - 296
EP - 306
JO - Calcified Tissue International
JF - Calcified Tissue International
IS - 3
ER -
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