The S haplotype-specific F-box protein gene, SFB, is defective in self-compatible haplotypes of Prunus avium and P. mume

Koichiro Ushijima, Hisayo Yamane, Akiko Watari, Eiko Kakehi, Kazuo Ikeda, Nathanael R. Hauck, Amy F. Iezzoni, Ryutaro Tao

研究成果査読

233 被引用数 (Scopus)

抄録

Many Prunus species, including sweet cherry and Japanese apricot, of the Rosaceae, display an S-RNase-based gametophytic self-incompatibility (GSI). The specificity of this outcrossing mechanism is determined by a minimum of two genes that are located in a multigene complex, termed the S locus, which controls the pistil and pollen specificities. SFB, a gene located in the S locus region, encodes an F-box protein that has appropriate S haplotype-specific variation to be the pollen determinant in the self-incompatibility reaction. This study characterizes SFBs of two self-compatible (SC) haplotypes, S 4′ and Sf, of Prunus. S4′ of sweet cherry is a pollen-part mutant (PPM) that was produced by X-ray irradiation, while Sf of Japanese apricot is a naturally occurring SC haplotype that is considered to be a PPM. DNA sequence analysis revealed defects in both SFB4′ and SFBf. A 4 bp deletion upstream from the HVa coding region of SFB4′ causes a frame-shift that produces transcripts of a defective SFB lacking the two hypervariable regions, HVa and HVb. Similarly, the presence of a 6.8 kbp insertion in the middle of the SFBf coding region leads to transcripts for a defective SFB lacking the C-terminal half that contains HVa and HVb. As all reported SFBs of functional S haplotypes encode intact SFB, the fact that the partial loss-of-function mutations in SFB are present in SC mutant haplotypes of Prunus provides additional evidence that SFB is the pollen S gene in GSI in Prunus.

本文言語English
ページ(範囲)573-586
ページ数14
ジャーナルPlant Journal
39
4
DOI
出版ステータスPublished - 8月 1 2004

ASJC Scopus subject areas

  • 遺伝学
  • 植物科学
  • 細胞生物学

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