Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis

Taishi Umezawa, Naoyuki Sugiyama, Masahide Mizoguchi, Shimpei Hayashi, Fumiyoshi Myouga, Kazuko Yamaguchi-Shinozaki, Yasushi Ishihama, Takashi Hirayama, Kazuo Shinozaki

研究成果査読

812 被引用数 (Scopus)

抄録

Abscisic acid (ABA) signaling is important for stress responses and developmental processes in plants. A subgroup of protein phosphatase 2C (group A PP2C) or SNF1-related protein kinase 2 (subclass III SnRK2) have been known as major negative or positive regulators of ABA signaling, respectively. Here, we demonstrate the physical and functional linkage between these two major signaling factors. Group A PP2Cs interacted physically with SnRK2s in various combinations, and efficiently inactivated ABA-activated SnRK2s via dephosphorylation of multiple Ser/Thr residues in the activation loop. This step was suppressed by the RCAR/PYR ABA receptors in response to ABA. However the abi1-1 mutated PP2C did not respond to the receptors and constitutively inactivated SnRK2. Our results demonstrate that group A PP2Cs act as 'gatekeepers' of subclass III SnRK2s, unraveling an important regulatory mechanism of ABA signaling.

本文言語English
ページ(範囲)17588-17593
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
106
41
DOI
出版ステータスPublished - 10月 13 2009
外部発表はい

ASJC Scopus subject areas

  • 一般

フィンガープリント

「Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル