TY - JOUR
T1 - Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death
AU - Takeichi, Sanae
AU - Nakajima, Yasuhiro
AU - Yukawa, Nobuhiro
AU - Fujita, Masaki Q.
AU - Saito, Takeshi
AU - Satoh, Fumiko
AU - Seto, Yoshihisa
AU - Kusakabe, Takahiko
AU - Jin, Zheng Bin
AU - Hasegawa, Iwao
AU - Nakano, Takamitsu
AU - Saniabadi, Abby
AU - Adachi, Masakazu
AU - Ohara, Naoki
AU - Usui, Shinichi
AU - Okazaki, Mitsuyo
AU - Nakajima, Katsuyuki
PY - 2004/5/1
Y1 - 2004/5/1
N2 - Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y=1.03x+229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.
AB - Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y=1.03x+229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.
KW - Free glycerol
KW - HPLC
KW - Postmortem plasma
KW - Remnant lipoproteins
KW - Sudden coronary death
KW - Triglycerides
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U2 - 10.1016/j.cccn.2003.12.031
DO - 10.1016/j.cccn.2003.12.031
M3 - Article
C2 - 15115680
AN - SCOPUS:11144357860
SN - 0009-8981
VL - 343
SP - 93
EP - 103
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -